General Information

ATG4 is a mammalian cysteine protease that plays a dual role in both the conjugation and deconjugation of ATG8 orthologs. It cleaves at the highly conserved C-terminal glycine of ATG8 orthologs, facilitating their conjugation with the phosphatidylethanolamine (PE) lipid. At least four mammalian homologs of the ATG4 protein have been identified where only, ATG4A and ATG4B demonstrated to cleave ATG8 orthologs. Specifically, ATG4A exhibits a primary cleavage preference for GATE16, followed by MAP1LC3A and GABARAP (PMID: 12473658, 15169837, 21177865).

Sequence 3D-Structure Experimental Structures

Monomer

AlphaFold Structures